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RT : This from is the best cartogram of the US I've seen. How we portray the US shapes our political imaginatio…
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2 days ago
Twitter
California's Deadly Wildfires: The Worst Is Yet to Come
"Forest fires might be seen as the particularly horrific…
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2 days ago
Twitter
RT : .: "I will always wonder about what led so many women, in particular, to reject an exceptionally qual…
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2 days ago
Twitter
RT : Just to put that in perspective... Wrigley Field in Chicago only holds 41,000 people. So imagine all of the fans an…
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2 days ago
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RT : The 92 year old Queen Elizabeth II of England out in the rain tonight attending a ceremony to commemorate the 100th…
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2 days ago
Twitter
RT : You can’t argue with Existentialist Airport Menu iPad.
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2 days ago
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RT : BREAKING: 's Secretary of State office has just REMOVED tons of public data from its website

They've r…
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3 days ago
Twitter
RT : Voter suppression is as fundamental to American politics as voting. Elections have two contests. The contest for vo…
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3 days ago
Twitter
RT : It turns out that Chatham County GA reported no mail in ballots. This is a fucking fiasco in my state. Do we need t…
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3 days ago
Twitter
Now scientists fear that deforestation and climate change are pushing the forest to a tipping point beyond which it…
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3 days ago
Twitter
RT : He's a bad person so it's easy to focus on his laziness and selfishness but we probably consistently underestimate…
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4 days ago
Twitter
RT : This is really cool — a simple app that’s an example of face detection & facial recognition using machine l…
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4 days ago
Twitter
RT : EXCLUSIVE: Ex-FBI Director James Comey used his private Gmail account hundreds of times to conduct government busin…
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4 days ago
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RT : I had never heard of a fire tornado until today and I really kind of hope I never see a firenado again in my life.…
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4 days ago
Twitter
RT : In today’s ⁦⁩: When received the leaders of , and , he began by blaming…
Lithuania  Trump  Estonia  Latvia  from twitter
4 days ago
Twitter
RT : We knew Whitaker was a critic of Russia inquiry but turns out to be more: in July 2017 he interviewed with WH Couns…
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4 days ago
Jamil Smith on Twitter: "“I am only five generations removed from the last known member of my family to be enslaved...And I carry that knowledge with me, because I owe it to him to cover the presidency the best way I know how, no matter how much pushbac

“I am only five generations removed from the last known member of my family to be enslaved...And I carry that knowledge with me, because I owe it to him to cover the presidency the best way I know how, no matter how much pushback I get.” Read @AprilDRyan. https://wapo.st/2T1uD95
4 days ago
Twitter
RT : Un spite of all our warnings the final attack on Hodeida is on. It will break the lifeline to millions and make th…
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4 days ago
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RT : Sisters — and those who love us, Please read this full thread by the President of the NAACP Legal Defense Fund.…
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4 days ago
Twitter
RT : The troubleshooting section for our new fridge has melted my heart. Imagine being the copywriter who sat there for…
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4 days ago
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RT : New on the new faces in Congress
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4 days ago
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RT : The Thousand Oaks shooter used a high capacity magazine that California voters banned anyone in the state from poss…
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4 days ago
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RT : NEW: Federal prosecutors have gathered evidence of Trump's participation in hush-money transactions that violated c…
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4 days ago
Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

Over the past few years, CRISPR-Cas technologies have emerged as powerful tools to edit genomes and modulate gene expression. They have been applied to perform high-throughput genetic screens with the purpose to understand the function of genes in a systematic manner, but the application of these screens to bacteria have so far remained limited. Here, we present the use of a library of ~92,000 guide RNAs directing the dCas9 protein to silence one by one all the genes in the chromosome of E. coli. To benchmark our method, we first investigate the performance of the technique to identify essential genes, highlighting several non-essential genes also found to be essential by other methods. We then apply our method to detect bacterial genes required by three different bacteriophages to kill E. coli and for the production of functional progeny by phage λ. Our screens highlight previously known and new genetic interactions between phages and their host’s pathways and emphasize the importance of bacterial capsule in the resistance to multiple phages. Altogether, our results demonstrate the usefulness of genome-wide CRISPR-dCas9 screens in bacteria to uncover genes involved in various phenotypes.
4 days ago
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