lung   284

« earlier    

JCI - Modeling metastasis biology and therapy in real time in the mouse lung
Lung slice culture - use agar insufflation

Pulmonary metastasis remains the leading ca use of death for cancer patients. Opportunities to improve treatment outcomes for patients require new methods to study and view the biology of metastatic progression. Here, we describe an ex vivo pulmonary metastasis assay (PuMA) in which the metastatic progression of GFP-expressing cancer cells, from a single cell to the formation of multicellular colonies, in the mouse lung microenvironment was assessed in real time for up to 21 days. The biological validity of this assay was confirmed by its prediction of the in vivo behavior of a variety of high- and low-metastatic human and mouse cancer cell lines and the discrimination of tumor microenvironments in the lung that were most permissive to metastasis. Using this approach, we provide what we believe to be new insights into the importance of tumor cell interactions with the stromal components of the lung microenvironment. Finally, the translational utility of this assay was demonstrated through its use in the evaluation of therapeutics at discrete time points during metastatic progression. We believe that this assay system is uniquely capable of advancing our understanding of both metastasis biology and therapeutic strategies.
lung  slices  in_vitro  Metastasis 
8 weeks ago by Segalllab
Lung Single-Cell Signaling Interaction Map Reveals Basophil Role in Macrophage Imprinting - ScienceDirect
lung basophils can polarize macrophages to M2

Lung development and function arises from the interactions between diverse cell types and lineages. Using single-cell RNA sequencing (RNA-seq), we characterize the cellular composition of the lung during development and identify vast dynamics in cell composition and their molecular characteristics. Analyzing 818 ligand-receptor interaction pairs within and between cell lineages, we identify broadly interacting cells, including AT2, innate lymphocytes (ILCs), and basophils. Using interleukin (IL)-33 receptor knockout mice and in vitro experiments, we show that basophils establish a lung-specific function imprinted by IL-33 and granulocyte-macrophage colony-stimulating factor (GM-CSF), characterized by unique signaling of cytokines and growth factors important for stromal, epithelial, and myeloid cell fates. Antibody-depletion strategies, diphtheria toxin-mediated selective depletion of basophils, and co-culture studies show that lung resident basophils are important regulators of alveolar macrophage development and function. Together, our study demonstrates how whole-tissue signaling interaction map on the single-cell level can broaden our understanding of cellular networks in health and disease.
macrophage_polarization  M2_macrophages  lung  basophils 
10 weeks ago by Segalllab
Interstitial Lung Abnormality
An ILA refers to a subtle or mild parenchymal abnormality identified in more than 5% of lungs on CT scans in patients in whom interstitial lung disease was not previously clinically suspected
lung  chest  ct 
january 2019 by observer1959
Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers | JCB
Partial characterizaiton of multiple types of endothelium that was purchased using nanostring for 283 genes. Also single cell RNAseq for lung so lung endothelium at greater depth

Through multiple cell–cell and cell–matrix interactions, epithelial and endothelial sheets form tight barriers. Modulators of the cytoskeleton contribute to barrier stability and act as rheostats of vascular permeability. In this study, we sought to identify cytoskeletal regulators that underlie barrier diversity across vessels. To achieve this, we correlated functional and structural barrier features to gene expression of endothelial cells (ECs) derived from different vascular beds. Within a subset of identified candidates, we found that the guanosine nucleotide exchange factor Vav3 was exclusively expressed by microvascular ECs and was closely associated with a high-resistance barrier phenotype. Ectopic expression of Vav3 in large artery and brain ECs significantly enhanced barrier resistance and cortical rearrangement of the actin cytoskeleton. Mechanistically, we found that the barrier effect of Vav3 is dependent on its Dbl homology domain and downstream activation of Rap1. Importantly, inactivation of Vav3 in vivo resulted in increased vascular leakage, highlighting its function as a key regulator of barrier stability.
endothelium  gene_expression  single_cell_rnaseq  lung 
january 2019 by Segalllab
Amphiregulin-Producing Pathogenic Memory T Helper 2 Cells Instruct Eosinophils to Secrete Osteopontin and Facilitate Airway Fibrosis: Immunity
Osteopontin production is also correlated with worse outcome in breast cancer - possible mechanism for areg to increase metastasis through fibrosis driven by areg stimulation of eosinophils?


We found that interleukin-33 (IL-33) enhanced amphiregulin production by the IL-33 receptor, ST2hi memory T helper 2 (Th2) cells. Amphiregulin-epidermal growth factor receptor (EGFR)-mediated signaling directly reprogramed eosinophils to an inflammatory state with enhanced production of osteopontin, a key profibrotic immunomodulatory protein. IL-5-producing memory Th2 cells and amphiregulin-producing memory Th2 cells appeared to cooperate to establish lung fibrosis. The analysis of polyps from patients with eosinophilic chronic rhinosinusitis revealed fibrosis with accumulation of amphiregulin-producing CRTH2hiCD161hiCD45RO+CD4+ Th2 cells and osteopontin-producing eosinophils. Thus, the IL-33-amphiregulin-osteopontin axis directs fibrotic responses in eosinophilic airway inflammation and is a potential target for the treatment of fibrosis induced by chronic allergic disorders.
AREG  fibrosis  lung  eosinophil  osteopontin 
december 2018 by Segalllab
How to Increase Lung Capacity: Breathing Exercises
COPD can significantly reduce lung function. Learn how to increase lung capacity with these easy breathing exercises.
breathing  techniques  lung  exercises 
october 2018 by thejaymo
A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte | Nature
have rnaseq for different categories - kif9 up in ciliated and one or two others


here we perform single-cell profiling of human bronchial epithelial cells and mouse tracheal epithelial cells to obtain a comprehensive census of cell types in the conducting airway and their behaviour in homeostasis and regeneration. Our analysis reveals cell states that represent known and novel cell populations, delineates their heterogeneity and identifies distinct differentiation trajectories during homeostasis and tissue repair. Finally, we identified a novel, rare cell type that we call the ‘pulmonary ionocyte’, which co-expresses FOXI1, multiple subunits of the vacuolar-type H+-ATPase (V-ATPase) and CFTR, the gene that is mutated in cystic fibrosis. Using immunofluorescence, modulation of signalling pathways and electrophysiology, we show that Notch signalling is necessary and FOXI1 expression is sufficient to drive the production of the pulmonary ionocyte, and that the pulmonary ionocyte is a major source of CFTR activity in the conducting airway epithelium.
lung  multciliated_cells  kif9 
august 2018 by Segalllab
Pazienti con la mutazione Ros-1, supereroi in cerca di una cura - Repubblica.it
Da un gruppo di pazienti con la mutazione Ros-1 è nato negli Stati Uniti e – grazie ai social network – si sta diffondendo in tutto il mondo, Italia incluso, un network di pazienti disposti a fornire campioni del loro tessuto per una sperimentazione clinica. E’ il primo caso di ricerca che nasce direttamente dai pazienti
healthcare  cancer  lung  ROS-1  patient  counterpoint  social-net 
june 2018 by PieroRivizzigno

« earlier    

related tags

1999  3d-conv  9gag  abuse  addiction  adenocarcinoma  aerothorax  afl  after  ageing  ai  alcohol  alton  amphiregulin  anatomy  and  ao3  app  architecture  areg  asian  asthma  auscultation  author  autoimmune  axl  b12  baby  back  bader  bagpipe  basophils  big  blood  braised  breast  breath  breathing  bubble  budget  burnley  calmtechnology  camera  cancer  cancerous  care  career  carotenoid  cedricprice  cell  cervical  challenge  champion  chemokines  chemotherapy  chest  chronic  cigarette  cigrette  clean  collapsed  colon  colonel  comedy  contemplation  cood  copacetic  copd  corriere  counterpoint  course  cranecreator  crash  crisis  csf1  ct  cuba  curcumin  cxcr2  damage  deep-learning  delicious  demographic  dentist  design  developing  development  device  diacetyl  diet-related  diet  disease  diseases  e-cig  e-cigarette  e-cigarettes  e0771  endothelium  eosinophil  exercises  f1  fanfic  fibrosis  filipino  first  fish  five  fiveelement  flavor  flavors  fleischner  flow  for  former  forskning  fractured  frontier  funny  garlic  gastric  gene_expression  ginsburg  glitter  gluten_free  gmcsf  granby/little  grand  growths  guide  had  has  health  healthcare  heart  hearth  hispanic  hospital  human  image-analysis  imaging  immune  immunotherapy  in  in_vitro  inflammation  interesting  intervention  introspection  invasion  ios  iron  jane/wellesley  justice  kalanithi  kif9  last  lauda:  laurence/tharkay  laurence  leadership  leg  lien  lines  liver  lobby  lobbying  lobbyist  low-grade  lrrk2  lukas  luna  lungs  lymphangioleiomyomatosis  m2_macrophages  macrophage  macrophage_polarization  malignant  map  markets  martine_rothblatt  master  medical  melanoma  metastasis  months  multciliated_cells  multi-chapter  murine  murmers  nanny  nature  nelsonhealth  newyork  nhs  niki  nm  no  nodule  nonsmall  novels  nsfw  oap  observation  onion  osteopontin  out  paradox  parkinsons  patient  pediatric  pendle  perfusion  pf  pneumosparklyosis  policy  polio  polish  politicians  popcorn  population  pregnancy  premature  pressure  prevention  prognosis  proposal  public  publichealth  pulmonary  pw  radiationtherapy  radio  realestate  realness  religion  remkoolhas  remote  removed  representation  research  response  risk  rockliff  ros-1  rsv  ruth  screening  see  sensor  sick  single_cell_rnaseq  six  slash  slices  smoking  social-net  sounds  spending  spirometer  spirometry  state  sugar  supplements  surgery  system  tar  tea  teachers  techniques  temeraire  test  thommayne  tien  time  tobacco  towers  transplant  tregs  tumors  underwent  undo  unet  vaccine  vape  vaping  vapor  victim  video  vinegar  visit  visual  vitamin  waveform  webmd  who  william  wind  wip  woes  womenshistory  world  xray  zhao  zinzino 

Copy this bookmark:



description:


tags: