cryopreservation   20

The evaluation of the transport medium for extracted premolars prior to cryopreservation: an in vitro study | SpringerLink
Cryopreservation as decontamination; cryopreserving teeth for eventually transplanting them back the same person's mouth.
dentist  cryopreservation  teeth 
december 2018 by porejide
Fertility Preservation in Women | NEJM
> Even though cryopreservation of ovarian tissue for female patients with cancer remains experimental,2 for prepubertal girls at high risk for premature ovarian insufficiency, it is the only option. What needs to happen before ovarian-tissue cryopreservation for young girls with cancer at high risk for premature ovarian insufficiency becomes an established procedure? Although all female patients with cancer deserve counseling regarding future fertility prognosis, those at highest risk for premature ovarian insufficiency (e.g., as defined by the Edinburgh criteria) need to be identified before the initiation of treatment.3 The safety and efficacy of the procedure in immunocompromised and vulnerable young girls must be further defined.4 No live births have yet been reported from ovarian-tissue cryopreservation and transplantation from a prepubertal girl. However, cases of pubertal induction have been reported,5 which suggests that ovarian tissue will be a source of mature oocytes after orthotopic reimplantation.
cryopreservation  ovaries  tissue_engineering 
january 2018 by porejide
IVF Transfer of Fresh or Frozen Embryos in Women without Polycystic Ovaries — NEJM
No significant difference between fresh and frozen embryos (but non-significantly, slightly higher for frozen). > After the first completed cycle, ongoing pregnancy occurred in 142 of 391 women (36.3%) in the frozen-embryo group and in 135 of 391 (34.5%) in the fresh-embryo group (risk ratio in the frozen-embryo group, 1.05; 95% confidence interval [CI], 0.87 to 1.27; P=0.65). Rates of live birth after the first transfer were 33.8% and 31.5%, respectively (risk ratio, 1.07; 95% CI, 0.88 to 1.31).
IVF  cryopreservation 
january 2018 by porejide
About Us — Organ Preservation Alliance
Lots of people working on organ cryopreservation.
cryopreservation  organs 
march 2017 by porejide
Cryogenically freezing organs: Could it be possible? -
> "From my perspective, the ability to do tissue banking in the volumes that we report in the paper exists now," Bischof said. Tissue transplants include corneas, bone grafts, heart valves, veins/arteries and skin.
"In the US, there are an estimated 1.75 million tissue transplants per year, which is almost 4,800 tissue transplants each day, every day of the year," said Sarah Gray, a spokeswoman for the American Association of Tissue Banks. There is no waiting list for most tissue transplants, except pediatric heart valves, because tissue can be cryopreserved, in some cases up to 10 years, she noted.
biobank  transplant  organ  cryopreservation 
march 2017 by porejide
Generation Cryo: Fighting Death in the Frozen Unknown
[[Kenneth Hayworth, president and co-founder of the Brain Preservation Foundation and an expert in the burgeoning science of connectomics, is critical of Alcor’s micrographs.

“It seems true that they don’t have ice damage, and that they show dehydration, but they also show a range of other damage,” he tells Gizmodo. “There is a reason that their methods have never been published in a peer-reviewed journal—they would be laughed at. And this is the best that their method could produce in an ideal laboratory setting on an animal model?”

By comparison, Hayworth pointed to a recent breakthrough by cryobiologist Robert McIntyre and his colleagues at 21st Century Medicine, who used a chemical compound to turn a rabbit’s brain into a plastic-like substance, and then cool it down to -211 degrees Fahrenheit (-135 degrees Celsius). The new technique, called Aldehyde Stabilized Cryopreservation (ASC), not only won the Brain Preservation Foundation’s Small Mammal Prize, but also demonstrated the feasibility of near-perfect, long-term structural preservation of an intact mammalian brain. Hayworth says that chemical fixation, in conjunction with cryonics, is the future of brain preservation, and that Alcor has it all wrong. As Hayworth said at the time, “This is not your father’s cryonics.”

According to de Wolf, Alcor has solved the problem of brain shrinking through the use of blood brain barrier modifiers, and it’s currently doing research to see whether such agents produce superior electron micrographs without sacrificing viability.

“The odds of reanimation being successful are better today than they’ve ever been and are continuously getting better,” he says, “while the odds of coming back from burial, cremation or every other alternative remain the same.”

“What about cancer?” More proposes. “If someone says they’re going to do everything they can to beat their cancer, such as chemotherapy and experimental drugs, virtually no one questions it.” He says that people resort to extreme measures to prolong life, spending copious amounts of money on health care, and that most money is spent on the last stages of life. “Yet few argue against that.”]]
against  Alcor  cryopreservation  brain  philosophy  arguments 
september 2016 by dandv
Wood frog adaptations to overwintering in Alaska: new limits to freezing tolerance | Journal of Experimental Biology
> Glucose concentrations in liver, heart and leg muscle from naturally freezing wood frogs were much higher than levels measured in corresponding tissues from wood frogs frozen in the laboratory in this and in other studies (Fig. 5, Table 2). Despite being cooled at rates of 0.05 and 0.5°C h−1, slower than the rates observed under natural conditions (as high as 1.6°C h−1), Alaskan wood frogs frozen in the laboratory accumulated glucose to levels that were only 22–40% in liver, 6–31% in heart and 7–20% in thigh muscle compared with those in corresponding tissues in wood frogs that froze outdoors
wood_frog  cryopreservation 
july 2016 by porejide
Morphological, ultrastructural and functional imaging of frozen/thawed and vitrified/warmed human ovarian tissue retrieved from oncological patients. - PubMed - NCBI
> For each patient, three pieces from each ovary were collected at the time of laparoscopy (fresh tissue) and after storage (SF/RT or V/W) and processed for light microscopy (LM) and transmission electron microscopy (TEM), to assess the morphological and ultrastructural features of follicles and stroma, and for laser scanning confocal microscopy (LSCM), to determine the functional energetic/redox stroma status. The preservation status of SF/RT and V/W ovarian tissues was compared with that of fresh ones, as well as between them.
cryopreservation  microscopy  vitrification 
june 2016 by porejide
crypoprize | ABOUT
> Proposed several months ago, the purpose of the prize will be to award a minimum of fifty thousand dollars to any individual or group of individuals who are able to place certain mammalian organs at cryogenic temperatures and to transplant those organs for a period of nine months and to show, during that time period, proper clinical function of them. The organs in question are the heart, lung, kidney, liver and pancreas. Other organs may be the subject of research leading to the awarding of the prize if pre-approved by the Immortalist Society. Fundraising is underway now.
cryonics  cryopreservation  organ_transplant 
april 2016 by porejide
Direct Measurement of Water States in Cryopreserved Cells Reveals Tolerance toward Ice Crystallization: Biophysical Journal
Argues with some data that ice (re-)formation during rewarming is more damaging than ice formation during freezing.
march 2016 by porejide
Antifreeze polymer protects cells as they thaw | Chemistry World
> The polymer functions outside the cells, so it can be washed-off after thawing. This may explain its good compatibility with red blood cells. Up to 60% red blood cell recovery after freezing was observed during slow thawing when the new polymer was used, and this increased to 80% when the cells were thawed quickly. Notably, the polymer was capable of inhibiting ice recrystallisation by 50%.
cryopreservation  chemistry 
july 2015 by porejide
This is whole tooth preservation, for later implantation. Apparently wisdom teeth are preferred as they can be reshaped into the shape of other teeth for re-implantation. This is effectively giving you mouth a spare tire. While the cells in the tooth are ideally preserved, the aim is not preservation of stem cells specifically though. Uses a special magnetic freezer tech developed in japan to supercool then instantaneously freeze the teeth for reduced cell damage.
teeth  bank  japan  cryogenic  wisdom  tooth  storage  cryopreservation  dental  medicine  health  CAS  Delicious 
january 2011 by asteroza
Connecticut scientist leads the way in freezing coral to give it life later
Hagedorn's lab in Hawaii is the only one in the world dedicated to coral cryopreservation. With funding from the Smithsonian and NOAA, Hagedorn is racing to create frozen archives of live tissue before the endangered Elkhorn and Staghorn corals disappear.
hawaii  coral  cryopreservation  cryogenics  freezing  smithsonian  noaa  secore  marinebiology  bytemarkscafe  bc20091216 
december 2009 by hawaii

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